Determination of artesunate using reversed-phase HPLC at increased temperature and ELSD detection

作者：Gaudin Karen^*; Kauss Tina; Lagueny Anne Marie; Millet Pascal; Fawaz Fawaz; Dubost Jean Pierre

来源：Journal of Separation Science, 2009, 32(2): 231-237.

DOI：10.1002/jssc.200800437

• Artesunate
• Dihydroartemisinin
• FLSD
• Increased temperature HPLC
• Rectal formulations

Artesunate (ART) determination can be performed by evaporative light scattering detection with mobile phase composed of CH(3)CN/HCOOH 0.01 M (40:60 v/v; pH 2.85). Evaporative light scattering detection instead of ILTV detection allowed to improve the sensitivity and the 1,01). However, the evaporative light scattering detection response of dihydro-artemisinin appears weaker than for ART, whereas with UV detection the response of ART and dihydroartemisinin seemed similar. Constant analysis time was obtained on using the mobile phase with a flow rate of 0.5 mL/min and column temperature at 60 degrees C instead of 0.7 mL/min at room temperature. This led to less solvent consumption. Moreover, decrease in the flow rate and increase in the column temperature were advantageous for higher sensitivity with both evaporative light scattering detection and UV detection. ART determination in rectal gel and suppositories were compared with these different detection modes and similar results were obtained.

• 出版日期2009-1