Short-term increases in pressure and shear stress attenuate age-related declines in endothelial function in skeletal muscle feed arteries

作者：Seawright John W; Luttrell Meredith; Trache Andreea; Woodman Christopher R^*

来源：European Journal of Applied Physiology, 2016, 116(7): 1305-1311.

DOI：10.1007/s00421-016-3388-9

• Endothelial dysfunction
• ACh-induced dilation
• Fischer 344 rats
• Vascular smooth muscle
• Skeletal muscle feed arteries
• Aging

We tested the hypothesis that exposure to a short-term (1 h) increase in intraluminal pressure and shear stress (SS), to mimic two mechanical signals associated with a bout of exercise, improves nitric oxide (NO)-mediated endothelium-dependent dilation in aged soleus muscle feed arteries (SFA). In addition, we hypothesized that pressure and SS would interact to produce greater improvements in endothelial function than pressure alone.
SFA from young (4 months) and old (24 months) Fischer 344 rats were cannulated and pressurized at 90 (P90) or 130 (P130) cmH(2)O and exposed to no SS (0 dyn/cm(2)) or high SS (similar to 65 dyn/cm(2)) for 1 h. At the end of the 1 h treatment period, pressure in all P130 SFA was set to 90 cmH(2)O and no SS (0 dyn/cm(2)) for examination of endothelium-dependent [flow and acetylcholine (ACh)] and endothelium-independent [sodium nitroprusside (SNP)] dilation. To evaluate the contribution of NO, vasodilator responses were assessed in the presence of N-omega-nitro- (l) -arginine (L-NNA).
Flow- and ACh-induced dilations were impaired in Old P90 SFA. Treatment with increased pressure + SS for 1 h improved flow- and ACh-induced dilations in old SFA. The beneficial effect of pressure + SS was abolished in the presence of L-NNA and was not greater than treatment with increased pressure alone.
These results indicate that short-duration increases in pressure + SS improve NO-mediated endothelium-dependent dilation in aged SFA; however, pressure and SS do not interact to produce greater improvements in endothelial function than pressure alone.

• 出版日期2016-7