Analysis of microbial diversity of inocula used in a five-face parallelepiped and standard microbial fuel cells

作者：Ortega Martinez A C; Juarez Lopez K; Solorza Feria O; Ponce Noyola M T; Galindez Mayer J; Rinderknecht Seijas N; Poggi Varaldo H M^*

来源：International Journal of Hydrogen Energy, 2013, 38(28): 12589-12599.

DOI：10.1016/j.ijhydene.2013.02.023

• Enriched inoculum
• Internal resistance
• Microbial fuel cell

This work had a double purpose: (i) to study the effect of sulphate-reducing (SR-In) and enriched (E-In) inocula on the characteristics of one-chamber standard microbial fuel cell (MFC-S) and parallelepiped cell and (ii) to analyze the bacterial communities in cells operated with either SR-In or E-In. The MFC-S of 150 mL consisted of one-chamber plexiglass cell with electrodes separated 7.8 cm. The MFC-P consisted of a parallelepiped built in plexiglass with a liquid volume of 270 mL. Five faces of this cell were fitted with %26apos;sandwich%26apos; cathode-membrane-anode assemblages (CMA). The values of internal resistance (R-int) were 4602 and 687 Omega, for the MFC-S loaded with SR-In and E-In, respectively. The values of R-int were 400 and 84, and 292 and 80 Omega for the faces connected in series and parallel and the MFC-P loaded with SR-In and E-In, respectively. Parallel connection of cell faces also significantly improved the electrochemical characteristics of the P cell (higher powers). In general, use of E-In in both types of MFC lead to improved power densities compared to SR-In. Molecular biology analysis of microbial communities showed that the E-In was less diverse than SR-In (in terms of phyla). An electrochemically active bacterium Geovibrio ferrireducens belonging to phylum Deferribacteres was found in the E-In. Predominance of Deferribacteres was observed in the E-In. Members of this phylum were not found in the SR-In.

• 出版日期2013-9-19