Analysis of heat-induced changes in protein expression of Stenotrophomonas maltophilia K279a reveals a role for GroEL in the host-temperature adaptation

作者：De Carolis Elena; Posteraro Brunella; Florio Ada Rita; Colonna Bianca; Prosseda Gianni; Bugli Francesca; Lorenzetti Silvia Renzetti; Fiscarelli Ersilia; Inzitari Rosanna; Iavarone Federica; Castagnola Massimo; Fadda Giovanni; Sanguinetti Maurizio^*

来源：International Journal of Medical Microbiology, 2011, 301(4): 273-281.

DOI：10.1016/j.ijmm.2010.10.001

• S. maltophilia
• MALDI-TOF MS
• Temperature-dependent expression
• GroEL

Stenotrophomonas maltophilia is a microorganism of environmental and clinical importance as well as a frequent airway colonizer of cystic fibrosis (CF) individuals. We combined 2-DE and MALDI-TOF MS to profile the protein expression in S. maltophilia K279a, a completely sequenced clinical isolate, grown at 37 degrees C with respect to the strain grown at 26 degrees C. Among the proteins up-regulated at 37 degrees C, we identified GroEL, a molecular chaperone that mainly assist the folding and unfolding of proteins under both normal and stress conditions. A 2.4-kb groESL mRNA was detected independently by Northern blot analyses with a groES- and a groEL-specific probe, indicating that S. maltophilia groES and groEL form an operon. Primer extension analysis of S. maltophilia groESL done in Escherichia coli showed that 2 promoters, P sigma(32) and P sigma(70), were utilized under the heat-shock and normal condition, respectively, whereas S. maltophilia groEL was shown to act as a heat-shock gene at 37 degrees C, 42 degrees C, and, to a lesser extent, at 50 degrees C by real-time RT-PCR analyses. Finally, immunoblot analyses revealed that S. maltophilia GroEL strongly reacted with sera from CF patients chronically infected by the microorganism, but did not with sera from CF patients with sporadic infection or uninfected.

• 出版日期2011-4