A comparative study on the effect of Curcumin and Chlorin-p(6) on the diffusion of two organic cations across a negatively charged lipid bilayer probed by second harmonic spectroscopy

作者：Saini R K; Varshney G K; Dube A; Gupta P K; Das K^*

来源：Journal of Molecular Structure, 2014, 1074: 22-26.

DOI：10.1016/j.molstruc.2014.05.056

• Second harmonic spectroscopy
• Drug-liposome interaction
• Malachite Green
• LDS
• Curcumin
• Chlorin-p(6)

The influence of Curcumin and Chlorin-p(6) (Cp-6) on the real time diffusion kinetics of two organic cations, LDS (LDS-698) and Malachite Green (MG) across a negatively charged phospholipid bilayer is investigated by Second Harmonic (SH) spectroscopy. The diffusion time constant of LDS at neutral pH in liposomes containing either Curcumin or Cp-6 is significantly reduced, the effect being more pronounced with Curcumin. At acidic pH, the quantum of reduction in the diffusion time constant of MG by both the drugs was observed to be similar. The relative changes in the average diffusion time constants of the cations with increasing drug concentration at pH 5.0 and 7.4 shows a substantial pH effect for Curcumin induced membrane permeability, while a modest pH effect was observed for Cp-6 induced membrane permeability. Based on available evidence this can be attributed to the increased interaction between the drug and the polar head groups of the lipid at pH 7.4 where the drug resides closer to the lipid-water interface.

• 出版日期2014-9-25