High throughput sequencing analysis of Trypanosoma brucei DRBD3/PTB1-bound mRNAs

作者：Das Anish; Bellofatto Vivian; Rosenfeld Jeffrey; Carrington Mark; Romero Zaliz Rocio; del Val Coral; Estevez Antonio M^*

来源：Molecular and Biochemical Parasitology, 2015, 199(1-2): 1-4.

DOI：10.1016/j.molbiopara.2015.02.003

• mRNA translation
• RNA-binding proteins
• Trypanosoma brucei

Trypanosomes are early-branched eukaryotes that show an unusual dependence on post-transcriptional mechanisms to regulate gene expression. RNA-binding proteins are crucial in controlling mRNA fate in these organisms, but their RNA substrates remain largely unknown. Here we have analyzed on a global scale the mRNAs associated with the Trypanosoma brucei RNA-binding protein DRBD3/PTB1, by capturing ribonucleoprotein complexes using UV cross-linking and subsequent immunoprecipitation. DRBD3/PTB1 associates with many transcripts encoding ribosomal proteins and translation factors. Consequently, silencing of DRBD3/PTB1 expression altered the protein synthesis rate. DRBD3/PTB1 also binds to mRNAs encoding the enzymes required to obtain energy through the oxidation of proline to succinate. We hypothesize that DRBD3/PTB1 is a key player in RNA regulon-based gene control influencing protein synthesis in trypanosomes.

• 出版日期2015-2
• 单位rutgers