Application of Polyhydroxyalkanoates Nanoparticles as Intracellular Sustained Drug-Release Vectors

作者：Xiong Yu Cui; Yao Yong Chao; Zhan Xiao Yong; Chen Guo Qiang^*

来源：Journal of Biomaterials Science, Polymer Edition, 2010, 21(1): 127-140.

DOI：10.1163/156856209X410283

• Polyhydroxyalkanoates
• PHB
• nanoparticles
• drug delivery
• sustained release
• intracellular drug release
• macrophage

Polyhydroxybutyrate (PHB), co-polyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and polylactic acid (PLA) were used to prepare nanoparticles with average sizes of 160, 250 and 150 nm, respectively. A lipid-soluble colorant, rhodamine B isothiocyanate (RBITC), was employed to study drug-release behaviors from these nanoparticles. A high RBITC drug-loading efficiency of over 75% was achieved with all PHA nanoparticles prepared. Macrophage endocytosis led to an intracellular RBITC drug sustained release over a period of at least 20 days for PHB and PHBHHx nanoparticles, while PLA nanoparticles and free drug lasted only 15 days and a week, respectively. Polymer properties and particle sizes showed little effect on drug-release behavior. This study showed for the first time that PHB and PHBHHx can be used effectively to achieve intracellular controlled drug releases.

• 出版日期2010
• 单位汕头大学; 清华大学