科研之友
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摘要
Background: Biohydrogen production from formate may aid in efficient chemical and fuel production using H-2 as the energy carrier. Results: The hyperthermophile Pyrococcus furiosus was engineered to convert formate to H-2. Conclusion: An 18-gene cluster can be inserted into the P. furiosus chromosome for active production of a membrane-bound system. Significance: This work demonstrates the versatility of this model organism for metabolic engineering purposes. Biohydrogen gas has enormous potential as a source of reductant for the microbial production of biofuels, but its low solubility and poor gas mass transfer rates are limiting factors. These limitations could be circumvented by engineering biofuel production in microorganisms that are also capable of generating H-2 from highly soluble chemicals such as formate, which can function as an electron donor. Herein, the model hyperthermophile, Pyrococcus furiosus, which grows optimally near 100 degrees C by fermenting sugars to produce H-2, has been engineered to also efficiently convert formate to H-2. Using a bacterial artificial chromosome vector, the 16.9-kb 18-gene cluster encoding the membrane-bound, respiratory formate hydrogen lyase complex of Thermococcus onnurineus was inserted into the P. furiosus chromosome and expressed as a functional unit. This enabled P. furiosus to utilize formate as well as sugars as an H-2 source and to do so at both 80 degrees and 95 degrees C, near the optimum growth temperature of the donor (T. onnurineus) and engineered host (P. furiosus), respectively. This accomplishment also demonstrates the versatility of P. furiosus for metabolic engineering applications.
- 出版日期2014-1-31
