Antiviral efficacy against hepatitis B virus replication of oleuropein isolated from Jasminum officinale L. var. grandiflorum

作者：Zhao Guiqin; Yin Zhifeng; Dong Junxing^*

来源：Journal of Ethnopharmacology, 2009, 125(2): 265-268.

DOI：10.1016/j.jep.2009.06.030

• Oleuropein
• Jasminum officinale L. var. grandiflorum
• Anti-HBV activity

Ethnopharmacological relevance: Jasminum officinale L var. grandiflorum (JOG) is a folk medicine used for the treatment of hepatitis in south of China. Phytochemical studies showed that secoiridoid glycosides are the typical constituents of this plant.
Aim of the study: The present study was undertaken to evaluate the effect of oleuropein (Ole) derived from the flowers of JOG on hepatitis B virus (HBV) replication in HepG2 2.2.15 cell line in vitro and duck hepatitis B virus (DHBV) replication in ducklings in vivo.
Material and methods: The extracellular hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) concentrations in cell culture medium were determined by ELISA. DHl3V in duck serum was analyzed by dot blot.
Results: Ole blocks effectively HBsAg secretion in HepG2 2.2.15 cells in a dose-dependent manner (IC(50) = 23.2 mu g/ml). Ole (80 mg/kg, intraperitoneally, twice daily) also reduced viremia in DHBV-infected ducks.
Conclusion: Ole therefore warrants further investigation as a potential therapeutic agent for HBV infection.

• 出版日期2009-9-7
• 单位承德医学院; 中国人民解放军军事医学科学院