A simple and rapid ultra-high-performance liquid chromatography-tandem mass spectrometry method to determine plasma biotin in hemodialysis patients

作者：Yagi Shigeaki^*; Nishizawa Manabu; Ando Itiro; Oguma Shiro; Sato Emiko; Imai Yutaka; Fujiwara Masako

来源：Biomedical Chromatography, 2016, 30(8): 1285-1290.

DOI：10.1002/bmc.3680

• biotin
• UHPLC-MS
• MS
• human plasma
• hemodialysis

A simple, rapid, and selective method for determination of plasma biotin was developed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). After single-step protein precipitation with methanol, biotin and stable isotope-labeled biotin as an internal standard (IS) were chromatographed on a pentafluorophenyl stationary-phase column (2.1x100mm, 2.7m) under isocratic conditions using 10mm ammonium formate-acetonitrile (93:7, v/v) at a flow rate of 0.6mL/min. The total chromatographic runtime was 5min for each injection. Detection was performed in a positive electrospray ionization mode by monitoring selected ion transitions at m/z 245.1/227.0 and 249.1/231.0 for biotin and the IS, respectively. The calibration curve was linear in the range of 0.05-2ng/mL using 300L of plasma. The intra- and inter-day precisions were all <7.1%. The accuracy varied from -0.7 to 8.2%. The developed UHPLC-MS/MS method was successfully applied to determine plasma biotin concentrations in hemodialysis patients.

• 出版日期2016-8