BACTERIAL EXPRESSION, PURIFICATION AND PARTIAL CHARACTERIZATION OF NEW RECOMBINANT CYSTEINE PROTEASE FROM MAIZE LEAVES: POST-TRANSCRIPTIONAL CHANGES UNDER OZONE STRESS

作者：Ahmad Rafiq^*; Zuily Fodil Yasmine; Passaquet Chantal; Ahmad Jam Nazeer

来源：Pakistan Journal of Botany, 2013, 45: 441-446.

Cysteine proteases are implicated in senescence, defense signaling pathways and cellular responses to biotic and abiotic stresses. In this context, we have cloned a novel cDNA encoding for papain family of cysteine protease from maize leaves. Mature part of papain-like protease was expressed in Escherichia coli using T7 promoter system. The recombinant protein was purified from inclusion bodies, refolded, characterized and used to produce corresponding antibodies in order to study post-transcriptional level of this specific protease under ozone stress. The results showed that ozone enhanced significantly papain-like cysteine protease at post-transcriptional level in 12th and 10th leaves of field grown maize plants. Simultaneously, senescence induced a rise in cysteine protease activity in both leaves. All together, these results suggest that ozone stress stimulates senescence processes, such as those related to proteolysis.

• 出版日期2013-1