Dynamic in situ chromosome immobilisation and DNA extraction using localized poly(N-isopropylacrylamide) phase transition

作者：Eriksen Johan^*; Thilsted Anil Haraksingh; Marie Rodolphe; Luscher Christopher James; Nielsen Lars Bue; Svendsen Winnie Edith; Szabo Peter; Kristensen Anders

来源：Biomicrofluidics, 2011, 5(3): 031101.

DOI：10.1063/1.3637631

• biochemistry
• bioMEMS
• cellular biophysics
• DNA
• enzymes
• hydrogels
• lab-on-a-chip
• microfluidics
• molecular biophysics

A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel.

• 出版日期2011-9