Alterations of Murine Subbasal Corneal Nerves After Environmental Dry Eye Stress

作者：Simsek Cem; Kojima Takashi^*; Dogru Murat; Tsubota Kazuo

来源：Investigative Ophthalmology & Visual Science, 2018, 59(5): 1986-1995.

DOI：10.1167/iovs.17-23743

• corneal subbasal nerves
• environmental stress
• dry eye
• confocal microscopy

PURPOSE. To investigate the morphologic changes in the corneal subbasal nerve (CSN) plexus in wild-type mice after exposure to environmental dry eye stress (EDES) using in vivo confocal microscopy (IVCM).
METHODS. We examined 22 eyes of 8-week-old wild-type male mice (Balb/c, n = 11). The mice were exposed to an air fan inside a small compartment 5 hours/day for 3 days (EDES). Aqueous tear secretion and corneal epithelial damage were assessed. The CSNs were investigated by laser-scanning IVCM. Density; tortuosity; and reflectivity of CSNs; and dendritic cell (DC) densities were evaluated using semi-automated NeuronJ software.
RESULTS. EDES significantly decreased the aqueous tear secretion quantity (P = 0.0019) and significantly increased the corneal fluorescein (P = 0.005) and lissamine green staining scores (P = 0003). The CSN density showed a significant decrease after EDES exposure (before, 2813 6 762 pixels/frame; after, 1906 6 896 pixels/frame, P = 0.0071). The tortuosity and the reflectivity grades did not show statistically significant differences after EDES exposure (tortuosity, P = 0.307; reflectivity, P = 0.758). However, the mean DC density showed a significant increase after EDES exposure (before, 12.6 +/- 65.94 cells/mm(2); after, 15.93 +/- 5.30 cells/mm(2), P = 0.026).
CONCLUSIONS. Even short-term exposure to EDES induced alterations in the CSN plexus morphology including decreased subbasal corneal nerve density and increased amount of DCs in mice. The EDES mouse model is a promising model to study the ocular surface and corneal nerve changes associated with dry eye disease.

• 出版日期2018-4