科研之友
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摘要
1. Long-term potentiation (LTP) of synaptic transmission is the putative mechanism underlying learning and memory. Despite intensive study, it remains controversial whether LTP is expressed at it pre- or postsynaptic locus. A new approach was used to investigate this question at excitatory synapses from the medial perforant path (MPP) onto granule cells in the hippocampal dentate gyrus. The variance of the evoked synaptic amplitude was plotted against mean synaptic amplitude at several different Cd2+ concentrations. The slope of the variance-mean plot estimates the average amplitude of the response following the release of a single reside of transmitter (Q(av)). A presynaptic modulation should not affect Q(av) but a postsynaptic modulation should alter it.
2. The variance-mean technique was tested LS applying the analysis before and after three different synaptic modulations: (i) a reduction in Q(av), by the addition of the competitive antagonist, CNQX; (ii) a reduction in the average probability of transmitter release (P-av) by the addition of baclofen; and (iii) an increase in the number of active synaptic terminals (N) by increasing the stimulus strength. CNQX reduced the average synaptic amplitude and Q(av), to the same extent, consistent with a postsynaptic action. In contrast, neither a change in N nor P-av altered Q(av). This confirms that the variance-mean technique can distinguish between a pre- and a postsynaptic site of modulation.
3. Induction of LTP increased EPSC amplitude by 50 +/- 0.4% (n = 5) and, in the same cells, increased Q(av) by 47 +/- 0.6%. There was no significant difference between the increase in EPSC amplitude and the increase in Q(av). Thus, LTP of the MPP input to dentate granule cells can be explained by an increase in the postsynaptic response to transmitter.
- 出版日期1999-7-1
