An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 '-5 '-Linked RNA

作者：Zhou, Wenhu; Ding, Jinsong; Liu, Juewen^*

来源：ChemBioChem, 2016, 17(10): 890-894.

DOI：10.1002/cbic.201500690

• biosensors
• DNAzymes
• lanthanides
• phosphorothioate
• RNA

RNA can form two types of linkage. In addition to the predominant 3'-5' linkage, 2'-5'-linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that specifically cleaves 2'-5' RNA by using Ce3+ as the metal cofactor, but leaves the 3'-5' counterpart intact. This Ce5 DNAzyme requires trivalent light lanthanide ions and shows a rate of 0.16 min(-1) in the presence of 10 mm Ce3+; the activity decreases with heavier lanthanide ions. This is the fastest DNAzyme reported for this reaction, and it might enable applications in chemical biology. As a proof-of-concept, using this DNAzyme, the reactions between phosphorothioate-modified RNA and strongly thiophilic metals (Hg2+ and Tl3+) were studied as a function of pH.

• 出版日期2016-5-17
• 单位中南大学