Development of a test strip for rapid detection of lactoperoxidase in raw milk

作者：Che, Hong-xia; Tian, Bo; Bai, Li-na; Cheng, Li-ming; Liu, Li-li; Zhang, Xiao-na; Jiang, Zhan-mei^*; Xu, Xiao-xi

来源：Journal of Zhejiang University-Science B(Biomedicine & Biotechnology), 2015, 16(8): 672-679.

DOI：10.1631/jzus.B1400359

• Lactoperoxidase
• Test strip
• Rapid detection
• Raw milk
• 3,3 ',5,5 '-Tetramethylbenzidine (TMB)

Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3',5,5'-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1-2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.

• 出版日期2015-8
• 单位东北农业大学