Air Pollution Particulate Matter Alters Antimycobacterial Respiratory Epithelium Innate Immunity

作者：Rivas Santiago Cesar E; Sarkar Srijata; Cantarella Pasquale; Osornio Vargas Alvaro; Quintana Belmares Raul; Meng Qingyu; Kirn Thomas J; Strickland Pamela Ohman; Chow Judith C; Watson John G; Torres Martha; Schwander Stephan^*

来源：Infection and Immunity, 2015, 83(6): 2507-2517.

DOI：10.1128/IAI.03018-14

Inhalation exposure to indoor air pollutants and cigarette smoke increases the risk of developing tuberculosis (TB). Whether exposure to ambient air pollution particulate matter (PM) alters protective human host immune responses against Mycobacterium tuberculosis has been little studied. Here, we examined the effect of PM from Iztapalapa, a municipality of Mexico City, with aerodynamic diameters below 2.5 mu m(PM2.5) and 10 mu m(PM10) on innate antimycobacterial immune responses in human alveolar type II epithelial cells of the A549 cell line. Exposure to PM2.5 or PM10 deregulated the ability of the A549 cells to express the antimicrobial peptides human beta-defensin 2 (HBD-2) and HBD-3 upon infection with M. tuberculosis and increased intracellular M. tuberculosis growth (as measured by CFU count). The observed modulation of antibacterial responsiveness by PM exposure was associated with the induction of senescence in PM-exposed A549 cells and was unrelated to PM-mediated loss of cell viability. Thus, the induction of senescence and downregulation of HBD-2 and HBD-3 expression in respiratory PM-exposed epithelial cells leading to enhanced M. tuberculosis growth represent mechanisms by which exposure to air pollution PM may increase the risk of M. tuberculosis infection and the development of TB.

• 出版日期2015-6