Akt or phosphoinositide-3-kinase inhibition reverses cardio-protection in Toll-like receptor 2 deficient mice

作者：Mersmann Jan; Nguyen Tran; Latsch Kathrina; Habeck Katharina; Iskandar Franziska; Zimmermann Rene; Zacharowski Kai^*

来源：Resuscitation, 2012, 83(11): 1404-1410.

DOI：10.1016/j.resuscitation.2012.04.001

• Myocardial ischaemia
• Toll-like receptor
• Myocardial reperfusion
• PI3K/AKT signalling

Aim: Absence or inhibition of Toll-like receptor 2 (TLR2) signalling during murine myocardial ischaemia/reperfusion (MI/R) decreases myocardial necrosis and inflammation, thereby ameliorating cardiac dysfunction and improving survival. In the present study, we provide evidence for the involvement of the phosphoinositide-3-kinase/Akt pathway in TLR2-dependent reperfusion injury.
Methods: Adult male wild-type (WT) and TLR2(-/-) mice were subjected to myocardial ischaemia (30 min) and reperfusion (4 h). Animals were treated with phosphoinositide-3-kinase inhibitor wortmannin, Akt inhibitor V (triciribine), or vehicle 1 h prior to MI/R. Protein expression levels of Akt1 and phosphoinositide-3-kinase and their respective phosphorylated forms were determined by Western blot analysis. Myocardial necrosis was quantified after staining with the tetrazolium method and by troponin T plasma levels.
Results: TLR2(-/-) mice displayed significantly increased Akt and phospho-Akt levels compared to WT mice, whilst no significant difference in phosphoinositide-3-kinase expression and phosphorylation could be observed. TLR2(-/-) mice also showed a blunted myocardial necrosis, the extent of which inversely correlated with Akt expression and degree of phosphorylation. Pharmacological inhibition of both, phosphoinositide-3-kinase or Akt, reversed the cardioprotection observed in TLR2(-/-) mice, whilst no effect could be observed in WT mice.
Conclusion: Akt is an important mediator of cardioprotection in TLR2(-/-) animals during MI/R. The effect is, however, likely mediated by its genomic overexpression in the heart of TLR2(-/-) animals whilst Akt activation by phosphoinositide-3-kinase is unaltered.

• 出版日期2012-11