A novel microfluidic system for the rapid analysis of protein thermal stability

作者：Yang, Xin; Liu, Jia; Xie, Ye Lei; Wang, Yang; Ying, Hong; Wu, Qiong; Huang, Wei; Jenkins, Gareth^*

来源：Analyst, 2014, 139(11): 2683-2686.

DOI：10.1039/c4an00173g

• ABSORPTION
• DISEASES
• MUTANTS
• BINDING
• STATE
• PH

We describe a simple microfluidic device for the rapid analysis of protein thermal stability using a novel imaging method. The change in UV absorption upon thermal denaturation or aggregation of proteins is used to get a spatial image of proteins' folding or aggregation state along a linear temperature gradient.

• 出版日期2014
• 单位南京大学