Modified Recombinant Proteins Can Be Exported via the Sec Pathway in Escherichia coli

作者：Chen, Nan^*; Hong, Fu-Lin; Wang, Hai-Hong; Yuan, Qi-Hang; Ma, Wan-Yan; Gao, Xu-Na; Shi, Rui; Zhang, Rui-Juan; Sun, Chang-Sheng; Wang, Sheng-Bin

来源：PLos One, 2012, 7(8): e42519.

DOI：10.1371/journal.pone.0042519

• ACETYL-COA CARBOXYLASE
• ACYL CARRIER PROTEIN
• BACTERIAL CYTOPLASMIC MEMBRANE
• POSTTRANSLATIONAL MODIFICATION
• DOMAIN
• EXPRESSION
• BIOTINYLATION
• TRANSLOCATION
• BIOSYNTHESIS
• PURIFICATION

The correct folding of a protein is a pre-requirement for its proper posttranslational modification. The Escherichia coli Sec pathway, in which preproteins, in an unfolded, translocation-competent state, are rapidly secreted across the cytoplasmic membrane, is commonly assumed to be unfavorable for their modification in the cytosol. Whether posttranslationally modified recombinant preproteins can be efficiently transported via the Sec pathway, however, remains unclear. ACP and BCCP domain (BCCP87) are carrier proteins that can be converted into active phosphopantetheinylated ACP (holo-ACP) and biotinylated-BCCP (holo-BCCP) by AcpS and BirA, respectively. In the present study, we show that, when ACP or BCCP87 is fused to the C-terminus of secretory protein YebF or MBP, the resulting fusion protein preYebF-ACP, preYebF-BCCP87, preMBP-ACP or preMBP-BCCP87 can be modified and then secreted. Our data demonstrate that posttranslational modification of preYebF-ACP, preYebF-BCCP87 preMBP-ACP and preMBP-BCCP87 can take place in the cytosol prior to translocation, and the Sec machinery accommodates these previously modified fusion proteins. High levels of active holo-ACP and holo-BCCP87 are achieved when AcpS or BirA is co-expressed, especially when sodium azide is used to retard their translocation across the inner membrane. Our results also provide an alternative to achieve a high level of modified recombinant proteins expressed extracellularly.

• 出版日期2012-8-13
• 单位华南农业大学