Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

作者：Miyauchi Yusuke; Kourist Robert; Uemura Daisuke; Miyamoto Kenji^*

来源：Chemical communications, 2011, 47(26): 7503-7505.

DOI：10.1039/c1cc11953b

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

• 出版日期2011