Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

作者：Omura Keita; Aiba Yuichiro; Onoda Hiroki; Stanfield Joshua Kyle; Ariyasu Shinya; Sugimoto Hiroshi; Shiro Yoshitsugu; Shoji Osami^*; Watanabe Yoshihito^*

来源：Chemical Communications, 2018, 54(57): 7892-7895.

DOI：10.1039/c8cc02760a

Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

• 出版日期2018-7-21
• 单位RIKEN