Nerve Conduction Velocity Is Regulated by the Inositol Polyphosphate-4-Phosphatase II Gene

作者：Lemcke Susanne^*; Mueller Susen; Moeller Steffen; Schillert Arne; Ziegler Andreas; Cepok Kauffeld Sabine; Comabella Manuel; Montalban Xavier; Rulicke Thomas; Nandakumar Kutty Selva; Hemmer Bernhard; Holmdahl Rikard; Pahnke Jens; Ibrahim Saleh M

来源：American Journal Of Pathology, 2014, 184(9): 2420-2429.

DOI：10.1016/j.ajpath.2014.05.021

Impairment of nerve conduction is common in neurodegenerative and neuroinflammatory diseases such as multiple sclerosis (MS), and measurement of evoked potentials (visual, motor, or sensory) has been widely used for diagnosis and recently also as a prognostic marker for MS. We used a classical genetic approach to identify novel genes controlling nerve conduction. First, we used quantitative trait mapping in F-2 progeny of B10/SJL mice to identify EAE31, a Locus controlling latency of motor evoked potentials (MEPs) and clinical onset of experimental autoimmune encephalomyelitis. Then, by combining congenic mapping, in silico haplotype analyses, and comparative genomics we identified inositol polyphosphate-4-phosphatase, type II (Inpp4b) as the quantitative trait gene for EAE31. Sequence variants of Inpp4b (C/A, exon 13; A/C, exon 14) were identified as differing among multiple mouse strains and correlated with individual cortical MEP latency differences. To evaluate the functional relevance of the amino acid exchanges at positions S474R and H548P, we generated transgenic mice carrying the longer-latency allele (Inpp4b(474R/548P)) in the C57BL/63 background. Inpp4b(474R/548P) mice exhibited significantly longer cortical MEP Latencies (4.5 +/- 0.22 ms versus 3.7 +/- 0.13 ms; P = 1.04 x 10(-9)), indicating that INPP4B regulates nerve conduction velocity. An association of an INPP48 polymorphism (rs13102150) with MS was observed in German and Spanish MS cohorts (3676 controls and 911 cases) (P = 8.8 x 10(-3)).

• 出版日期2014-9
• 单位河北医科大学