Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots

作者：Kakizuka Taishi; Ikezaki Keigo; Kaneshiro Junichi; Fujita Hideaki; Watanabe Tomonobu M; Ichimura Taro^*

来源：Biomedical Optics Express, 2016, 7(7): 2475-2493.

DOI：10.1364/BOE.7.002475

Simultaneous nanometric tracking of multiple motor proteins was achieved by combining multicolor fluorescent labeling of target proteins and imaging spectroscopy, revealing dynamic behaviors of multiple motor proteins at the sub-diffraction-limit scale. Using quantum dot probes of distinct colors, we experimentally verified the localization precision to be a few nanometers at temporal resolution of 30 ms or faster. One-dimensional processive movement of two heads of a single myosin molecule and multiple myosin molecules was successfully traced. Furthermore, the system was modified for two-dimensional measurement and applied to tracking of multiple myosin molecules. Our approach is useful for investigating cooperative movement of proteins in supramolecular nanomachinery.

• 出版日期2016-7-1
• 单位RIKEN