Anti-complement sesquiterpenes from Viola yedoensis

作者：Du Dongsheng^*; Cheng Zhihong; Chen Daofeng

来源：Fitoterapia, 2015, 101: 73-79.

DOI：10.1016/j.fitote2014.12.015

• Anti-complement
• Sesquiterpenes
• Viola yedoensis
• Mosher's method

Two new germacrane sesquiterpenes, yedoensins A (1) and B (2), together with 8 known ones (3-10) were isolated from the herb of Viola yedoensis. The structures of the new compounds were established by extensive spectroscopic means including 1D (H-1 and C-13) and 2D NMR experiments (HSQC, HMBC, and NOESY) as well as HR-ESI-MS analysis. The absolute configurations of the known sesquiterpenes versicolactone B (3) and madolin W (6) were determined by a modified Masher's method for the first time. The sesquiterpenes 1-3, and 5-9 exhibited anticomplement activity against the classical pathway (CP) and the alternative pathway (AP) with the CH50 and AP(50) values ranging from 0.14 to 037 mg/mL and 032 to 0.54 mg/mL, respectively. Preliminary mechanism study using complement-depleted sera showed that yedoensin A (1) and versicolactone B (3) acted on C1q, C3 and C9, while madolin W (6), aristoyunnolin E (7) and madolin Y (9) interacted with C1q, C3, C5 and C9 components in the complement activation cascade.

• 出版日期2015-3
• 单位复旦大学