An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

作者:Burger R; Kurzbuch D; Gorkin R; Kijanka G; Glynn M; McDonagh C; Ducree J*
来源:Lab on a Chip, 2015, 15(2): 378-381.
DOI:10.1039/c4lc01002g

摘要

In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.

  • 出版日期2015