Association of IL1B polymorphisms with gastric cancer in a Chinese population

作者：Wang Weipeng; Ni Kunyi; Zhou Guohua^*

来源：Clinical Biochemistry, 2007, 40(3-4): 218-225.

DOI：10.1016/j.clinbiochem.2006.10.018

• risk association
• gastric cancer
• polymorphisms
• IL1B
• ALM-ASA

Objectives: To investigate the association between ILIB polymorphisms and risk of gastric cancer in a Chinese population, seven SNPs in the ILIB gene were selected for this study.
Methods: A multiplex genotyping method, which is based on adapter ligation and allele-specific amplification, was established to type seven SNPs on the ILIB gene simultaneously. One hundred and forty-one non-cancer outpatients and 97 patients with gastric cancer were genotyped, and the relation between ILIB polymorphisms and gastric cancer was statistically analyzed.
Results: The seven SNPs were successfully typed and the results were consistent with those obtained by both Sanger's sequencing and PCRRFLP. Handling with statistical analysis, we observed significantly different genotype frequencies of 0794C > T (chi(2)=6.11, P=0.05), 1274C > T (chi(2)=6.86, P=0.03) and 2143T > C (chi(2)=6.86, P=0.03) between patients and controls.
Conclusion: ALM-ASA is a potential method for multiplex SNP typing with a low consumption of genomic DNA. SNPs 0794C > T, 1274C > T, and 2143T > C are associated with gastric cancer.

• 出版日期2007-2
• 单位河北医科大学; 中国药科大学; 南京大学