Novel DNA polymerase mutations conferring cytomegalovirus resistance: Input of BAC-recombinant phenotyping and 3D model

作者：Hantz Sebastien; Cotin Sebastien; Borst Eva; Couvreux Anthony; Salmier Arielle; Garrigue Isabelle; Merville Pierre; Mengelle Catherine; Attal Michel; Messerle Martin; Alain Sophie^*

来源：Antiviral Research, 2013, 98(1): 130-134.

DOI：10.1016/j.antiviral.2013.02.002

• Cytomegalovirus
• Ganciclovir
• Cidofovir
• Resistance
• Transplant recipient
• BAC
• 3D-model

Long-term exposure to antiviral therapy in immunocompromised patients favors emergence of human cytomegalovirus (HCMV) resistance mutations. Two new UL54 DNA polymerase mutations (deletion of codon 524 and N408S substitution) identified in a kidney recipient and a bone marrow recipient respectively were characterized. Marker transfer experiment through recombination into a HCMV AD169 BAC demonstrated del524 and mutation N408S confer GCV and CDV resistance. These results suggest continued mutation of UL54 under selective antiviral pressure. Characterization of each new mutation is thus required to inform genotypic assays and to better understand the functional regions of UL54 for the development of novel antivirals.

• 出版日期2013-4