A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

作者:Lee Hye Jin; Kim Byoung Chan; Oh Min Kyu*; Kim Jungbae
来源:Chemical Communications, 2012, 48(48): 5971-5973.
DOI:10.1039/c2cc30710c

摘要

Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 degrees C for 66 days.

  • 出版日期2012