A Model for Protein Sequence Evolution Based on Selective Pressure for Protein Stability: Application to Hemoglobins

作者：Marsh Lorraine^*

来源：Evolutionary Bioinformatics, 2009, 5: 107-118.

DOI：10.4137/ebo.s3120

• protein stability
• negative selection
• protein structure
• evolutionary model

Negative selection against protein instability is a central influence on evolution of proteins. Protein stability is maintained over evolution despite changes in underlying sequences. An empirical all-site stability-based model of evolution was developed to focus on the selection of residues arising from their contributions to protein stability. In this model, site rates could vary. A structure-based method was used to predict stationary frequencies of hemoglobin residues based on their propensity to promote protein stability at a site. Sites with destabilizing residues were shown to change more rapidly in hemoglobins than sites with stabilizing residues. For diverse proteins the results were consistent with stability-based selection. Maximum likelihood studies with hemoglobins supported the stability-based model over simple Poisson-based methods. These observations are consistent with suggestions that purifying selection to maintain protein structural stability plays a dominant role in protein evolution.

• 出版日期2009