Schistosoma mansoni: Developmental arrest of miracidia treated with histone deacetylase inhibitors

作者：Azzi A; Cosseau C; Grunau C^*

来源：Experimental Parasitology, 2009, 121(3): 288-291.

DOI：10.1016/j.exppara.2008.11.010

• Platyhelminth
• Schistosoma mansoni
• Histone deacetylase
• Trichostatin A
• Metamorphosis
• Development

In the present study, we examined the effect of the histone deacetylase (HDAC) inhibitors trichostatin A (TSA), valproic acid (VA) and sodium-butyrate on the metamorphosis of larvae of the human blood-fluke Schistosoma mansoni from the free-swimming miracidia into the intramolluskal sporocyst. We show that HDAC inhibitors block transformation in concentration dependant manner. TSA reversibly blocks this developmental process: only 13 +/- 11% of TSA treated miracidia transform into sporocysts in-vitro, compared to 92 +/- 3% in the mock-treated control. Other enzyme inhibitors such as cycloheximide or hydroxyurea had no effect on metamorphosis. For treatment of up to 4 h, the effect of TSA was completely reversible. Our data indicates that HDAC activity is necessary for the transformation of S. mansoni miracidia during infection of the snail host.

• 出版日期2009-3