A medium-chain fatty acid as an alternative energy source in mouse preimplantation development

作者：Yamada Mitsutoshi; Takanashi Kazumi; Hamatani Toshio^*; Hirayama Akiyoshi; Akutsu Hidenori; Fukunaga Tomoko; Ogawa Seiji; Sugawara Kana; Shinoda Kosaku; Soga Tomoyoshi; Umezawa Akihiro; Kuji Naoaki; Yoshimura Yasunori; Tomita Masaru

来源：Scientific Reports, 2012, 2(1): 930.

DOI：10.1038/srep00930

To further optimize the culturing of preimplantation embryos, we undertook metabolomic analysis of relevant culture media using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). We detected 28 metabolites: 23 embryo-excreted metabolites including 16 amino acids and 5 media-derived metabolites (e.g., octanoate, a medium-chain fatty acid (MCFA)). Due to the lack of information on MCFAs in mammalian preimplantation development, this study examined octanoate as a potential alternative energy source for preimplantation embryo cultures. No embryos survived in culture media lacking FAs, pyruvate, and glucose, but supplementation of octanoate rescued the embryonic development. Immunoblotting showed significant expression of acyl-CoA dehydrogenase and hydroxyacyl-CoA dehydrogenase, important enzymes for beta-oxidation of MCFAs, in preimplantation embryo. Furthermore, CE-TOFMS traced [1-C-13(8)] octanoate added to the culture media into intermediate metabolites of the TCA cycle via beta-oxidation in mitochondria. These results are the first demonstration that octanoate could provide an efficient alternative energy source throughout preimplantation development.

• 出版日期2012-12-5