Disruption of Spectrin-Like Cytoskeleton in Differentiating Keratinocytes by PKC delta Activation Is Associated with Phosphorylated Adducin

作者：Zhao Kong Nan^*; Masci Paul P; Lavin Martin F

来源：PLos One, 2011, 6(12): e28267.

DOI：10.1371/journal.pone.0028267

Spectrin is a central component of the cytoskeletal protein network in a variety of erythroid and non-erythroid cells. In keratinocytes, this protein has been shown to be pericytoplasmic and plasma membrane associated, but its characteristics and function have not been established in these cells. Here we demonstrate that spectrin increases dramatically in amount and is assembled into the cytoskeleton during differentiation in mouse and human keratinocytes. The spectrin-like cytoskeleton was predominantly organized in the granular and cornified layers of the epidermis and disrupted by actin filament inhibitors, but not by anti-mitotic drugs. When the cytoskeleton was disrupted PKC delta was activated by phosphorylation on Thr505. Specific inhibition of PKC delta((Thr505)) activation with rottlerin prevented disruption of the spectrin-like cytoskeleton and the associated morphological changes that accompany differentiation. Rottlerin also inhibited specific phosphorylation of the PKC delta substrate adducin, a cytoskeletal protein. Furthermore, knock-down of endogenous adducin affected not only expression of adducin, but also spectrin and PKC delta, and severely disrupted organization of the spectrin-like cytoskeleton and cytoskeletal distribution of both adducin and PKC delta. These results demonstrate that organization of a spectrin-like cytoskeleton is associated with keratinocytes differentiation, and disruption of this cytoskeleton is mediated by either PKC delta((Thr505)) phosphorylation associated with phosphorylated adducin or due to reduction of endogenous adducin, which normally connects and stabilizes the spectrin-actin complex.

• 出版日期2011-12-7
• 单位河北医科大学