Analysis of the V genes coding for a monospecific human antibody to myosin and functional expression of single chain Fv fragments

作者：Laroche Traineau J^*; Jacobin MJ; Biard Piechaczyk M; Vuillemin L; Chagnaud JL; Pau B; Nurden AT; Clofent Sanchez G

来源：FEBS Letters, 1999, 460(1): 86-92.

DOI：10.1016/S0014-5793(99)01308-3

• human antibody
• IgM
• immunoglobulin variable region
• single chain variable fragment
• myosin
• B cell repertoire

A monospecific human IgM monoclonal antibody (mAb), reactive,vith myosin from human heart, has been obtained by EBV transformation. This mAb mag have a diagnostic potential in the imaging of myocardial necrosis, However, owing to the fact that the molecular mass of an IgM is 900 kDa, a poor diffusion and a slow penetration inside necrotic myocytes could reduce its capacity for scintigraphic detection. In order to alleviate these problems, we constructed the scFv by cloning the VH and VL domains into the pHOG21 vector. Analysis of the V genes proved an unmutated configuration shelving that the immortalized B cell issued from the primary IgM repertoire. The expression product in Escherichia coli was a 35 kDa scFv fragment with the antigen-binding specificity of the parental mAb.

• 出版日期1999-10-22
• 单位河南工业大学