科研之友
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摘要
We have constructed an expression vector carrying the Escherichia coli his operon control region to study the ability of defined segments of DNA to cause rho factor-mediated transcription termination both in vivo and in vitro. We have previously identified a consensus motif consisting of a region of high cytosine over guanosine content common to several cryptic intracistronic transcription termination elements unmasked by polar mutations. We show that a DNA fragment possessing features similar to the ones previously identified is capable of causing rho-mediated release of transcripts in vivo and in vitro. The efficiency of termination depends on the length and relative cytosine over guanosine ratio of the element.
- 出版日期1991-12
