Preparation of monolithic microcolumn filled with quartz sand and its separation property of reversed-phase electrochromatography

作者：Deng N; He YZ^*; Wang XK; Wang L; Su QD

来源：Chemical Journal of Chinese Universities, 2005, 26(11): 2019-2022.

• monolithic column
• electrochromatography
• fine quartz sand

Capillary electrochromatography (CEC) possesses advantages of high separation efficiency and selectivity, but it shows some shortcomings, such as low sample loadability and restricted concentration detectability, due to its small inner diameter. In this paper, a silica gel monolithic microcolumn with high surface area was prepared by sol-gel polymerization of tetraethoxysilane under acidic conditions, which was filled with fine quartz sand in 2.2 mm i.d. fused silica tube. The experiment results indicate that the heating effect caused by electric current in the large diameter microcolumn was eliminated by packing fine quartz sand. With the mobile phase driven by electroosmotic flow, two aromatic compounds were separated successfully by the reversed-phase electrochromatography. The experiment results indicate that the microcolumn can be used for electrochramatography separation and its detection sensitivity compared with CEC can be improved.

• 出版日期2005-11-10
• 单位中国科学技术大学