Tombusviruses upregulate phospholipid biosynthesis via interaction between p33 replication protein and yeast lipid sensor proteins during virus replication in yeast

作者：Barajas Daniel; Xu Kai; Sharma Monika; Wu Cheng Yu; Nagy Peter D^*

来源：Virology, 2014, 471: 72-80.

DOI：10.1016/j.virol.2014.10.005

• FFAT domain
• VAP domain
• Phosphatidic acid
• Transcription repressor
• Phospholipids
• Yeast host
• Tomato bushy stunt virus
• Replication in vitro
• Replicase
• Membrane proliferation

Positive-stranded RNA viruses induce new membranous structures and promote membrane proliferation in infected cells to facilitate viral replication. In this paper, the authors show that a plant-infecting tombusvirus upregulates transcription of phospholipid biosynthesis genes, such as INO1, OPI3 and CHO1, and increases phospholipid levels in yeast model host. This is accomplished by the viral p33 replication protein, which interacts with Opi1p FFAT domain protein and Scs2p VAP protein. Opi1p and Scs2p are phospholipid sensor proteins and they repress the expression of phospholipid genes. Accordingly, deletion of OPI1 transcription repressor in yeast has a stimulatory effect on TBSV RNA accumulation and enhanced tombusvirus replicase activity in an in vitro assay. Altogether, the presented data convincingly demonstrate that de novo lipid biosynthesis is required for optimal TBSV replication. Overall, this work reveals that a (+)RNA virus reprograms the phospholipid biosynthesis pathway in a unique way to facilitate its replication in yeast cells.

• 出版日期2014-12