Building biological foundries for next-generation synthetic biology

作者：Chao Ran; Yuan YongBo; Zhao HuiMin^*

来源：Science China Life Sciences, 2015, 58(7): 658-665.

DOI：10.1007/s11427-015-4866-8

• biofabrication
• automation
• metabolic engineering
• biosystems design
• DNA assembly
• DNA sequencing
• metabolite analysis

Synthetic biology is an interdisciplinary field that takes top-down approaches to understand and engineer biological systems through design-build-test cycles. A number of advances in this relatively young field have greatly accelerated such engineering cycles. Specifically, various innovative tools were developed for in silico biosystems design, DNA de novo synthesis and assembly, construct verification, as well as metabolite analysis, which have laid a solid foundation for building biological foundries for rapid prototyping of improved or novel biosystems. This review summarizes the state-of-the-art technologies for synthetic biology and discusses the challenges to establish such biological foundries.

• 出版日期2015-7