Detection of Bacillus thuringiensis Cry1Ab protein based on surface plasmon resonance immunosensor

作者：Ming, Huami; Wang, Manli; Yin, Hongzong^*

来源：Analytical Biochemistry, 2015, 468: 59-65.

DOI：10.1016/j.ab.2014.09.014

• Surface plasmon resonance immunosensor
• Au/Ag alloy nanoparticles
• Protein A
• Bacillus thuringiensis Cry1Ab protein
• Transgenic crops

Two novel surface plasmon resonance immunosensors were fabricated for detection of the Bacillus thuringiensis Cry1Ab protein and to demonstrate their performance in analyzing Cry1Ab protein in crop samples. Sensor 2 was modified by 1,6-hexanedithiol, Au/Ag alloy nanoparticles, 3-mercaptopropionic acid, and protein A (or not [sensor 1]), with Cry1Ab monoclonal antibody. As a result, both of the immunosensors exhibited satisfactory linear responses in the Cry1Ab protein concentration ranges of 10 to 500 ng ml(-1) and 8 to 1000 ng ml(-1), and the detection limits were 5.0 and 4.8 ng ml(-1), respectively. The immunosensors possessed good specificity and acceptable reproducibility. In addition, crop samples could be analyzed after a simple treatment. The transgenic crops could be easily identified from the conventional ones by the two immunosensors.

• 出版日期2015-1-1
• 单位山东农业大学