Detection of a divergent variant of grapevine virus F by next-generation sequencing

作者：Molenaar Nicholas; Burger Johan T; Maree Hans J^*

来源：Archives of Virology, 2015, 160(8): 2125-2127.

DOI：10.1007/s00705-015-2466-3

The complete genome sequence of a South African isolate of grapevine virus F (GVF) is presented. It was first detected by metagenomic next-generation sequencing of field samples and validated through direct Sanger sequencing. The genome sequence of GVF isolate V5 consists of 7539 nucleotides and contains a poly(A) tail. It has a typical vitivirus genome arrangement that comprises five open reading frames (ORFs), which share only 88.96 % nucleotide sequence identity with the existing complete GVF genome sequence (JX105428).

• 出版日期2015-8