Anti-Neuroinflammatory Effects of Houttuynia cordata Extract on LPS-Stimulated BV-2 Microglia

作者：Park Tae Kyu; Koppula Sushruta; Kim Moo Sung; Jung Seung Hyo; Kang Hyun^*

来源：Tropical Journal of Pharmaceutical Research, 2013, 12(4): 523-528.

DOI：10.4314/tjpr.v12i4.12

• Houttuynia cordata
• DPPH radicals
• antioxidant
• neuroinflammation
• BV-2 cells
• iNOS
• COX-2
• IL-6

Purpose: To evaluate the anti-neuroinflammatory effects of Houttuynia cordata extract (H. cordata) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells, and its anti-oxidant properties. %26lt;br%26gt;Methods: Anti-oxidant properties were evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay. LPS was used to stimulate BV-2 cells. Nitric oxide (NO) levels were measured using Griess assay. Inducible NO synthase (iNOS) expression, interleukin (IL)-6 expressional level were determined by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. %26lt;br%26gt;Results: Ethyl actetae (HC-EA) extract of H. cordata significantly scavenged DPPH free radicals in a concentration-dependent fashion. The increased levels of NO, iNOS and IL-6 in LPS-stimulated BV-2 microglial cells were also suppressed by HC-EA extract in a concentration-dependent manner. %26lt;br%26gt;Conclusion: The result indicate that the HC-EA extract exhibited strong anti-oxidant properties and inhibited the excessive production of pro-inflammatory mediators, including NO, iNOS and IL-6, in LPS-stimulated BV-2 cells. The anti-oxidant phenolic compounds present in HC-EA extract might play an important role in ameliorating neuroinflammatory processes in LPS-stimulated BV-2 microglial cells.

• 出版日期2013-8