MS/MS-Free Protein Identification in Complex Mixtures Using Multiple Enzymes with Complementary Specificity

作者：Ivanov Mark V; Tarasova Irina A; Levitsky Lev I; Solovyeva Elizaveta M; Pridatchenko Marina L; Lobas Anna A; Bubis Julia A; Gorshkov Mikhail V^*

来源：Journal of Proteome Research, 2017, 16(11): 3989-3999.

DOI：10.1021/acs.jproteome.7b00365

• proteomics
• database search
• MS1-only search
• peptide mass fingerprinting
• shotgun proteomics
• protein identification

In this work, we present the results of evaluation of a workflow that employs a multienzyme digestion strategy for MS1-based protein identification in "shotgun" proteomic applications. In the proposed strategy, several cleavage reagents of different specificity were used for parallel digestion of the protein sample followed by MS1 and retention time (RT) based search. Proof of principle for the proposed strategy was performed using experimental data obtained for the annotated 48-protein standard. By using the developed approach, up to 90% of proteins from the standard were unambiguously identified. The approach was further applied to HeLa proteome data. For the sample of this complexity, the proposed MS1-only strategy determined correctly up to 34% of all proteins identified using standard MS/MS-based database search. It was also found that the results of MS1-only search were independent of the chromatographic gradient time in a wide range of gradients from 15-120 min. Potentially, rapid MS1-only proteome characterization can be an alternative or complementary to the MS/MS-based "shotgun" analyses in the studies, in which the experimental time is more important than the depth of the proteome coverage.

• 出版日期2017-11