An antifungal peptide from red lentil seeds

作者：Wang, H. X.^*; Ng, T. B.

来源：Peptides, 2007, 28(3): 547-552.

DOI：10.1016/j.peptides.2006.10.006

• antifungal peptide
• Lens culinaris
• isolation

An antifungal peptide, with a molecular mass of 11 kDa, was isolated from dry seeds of the red lentil (Lens culinaris) using a procedure that involved four chromatographic steps. The antifungal peptide was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and S-Sepharose. The final chromatographic step involved gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal peptide inhibited mycelial growth in Mycosphaerella arachidicola with an IC50 of 36 mu M. It also exhibited antifungal activity against Fusarium oxysporum, but there was no inhibitory activity toward tumor cell lines and human immunodeficiency virus type 1 reverse transcriptase (RT).

• 出版日期2007-3
• 单位香港中文大学; 中国农业大学; 农业生物技术国家重点实验室