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摘要
Objective: To evaluate the flow dynamics of dentine fluid using a chemiluminescence method in vitro. Materials and methods: Horizontally sliced coronal dentine specimens with thicknesses of 1.4, 1.6, 1.8, and 2.0 mm (n = 10 each) were prepared from extracted human third molars. After cleaning with EDTA, a mounted specimen was clamped between 2 acrylic chambers attached to both the occlusal and pulpal sides. The occlusal chamber, which was closed with a glass coverslip, was filled with a chemiluminescent solution (0.02% luminol and 1% sodium hydroxide in water). A trigger solution of 1% hydrogen peroxide and 1% potassium ferricyanide was injected into the pulpal chamber at a constant pressure of 2.5 kPa, and allowed to immediately flow into the patent dentinal tubules. Four consecutive measurements (T1-T4) were performed on each sample by recording the emission of chemiluminescence with a photodetector. The relationship between the crossing time of the liquid through the slice and dentine thickness was examined. Results: An apparent time delay was detected between the starting points of the trigger solution run and photochemical emission at T1. Dt (Dt, s) values of each thickness group were 13.6 +/- 4.25 for 1.4 mm, 18.1 +/- 2.38 for 1.6 mm, 28.0 +/- 2.46 for 1.8 mm, and 39.2 +/- 8.61 for 2.0 mm, respectively. Dt significantly decreased as dentine became thinner towards the pulp chamber (P < 0.001). Conclusions: The velocity of fluid flow increased both with increasing dentine depth or reduction of remaining dentine thickness.
- 出版日期2015-1
