Mitochondria Synthesize Melatonin to Ameliorate Its Function and Improve Mice Oocyte's Quality under in Vitro Conditions

作者：He, Changjiu; Wang, Jing; Zhang, Zhenzhen; Yang, Minghui; Li, Yu; Tian, Xiuzhi; Ma, Teng; Tao, Jingli; Zhu, Kuanfeng; Song, Yukun; Ji, Pengyun; Liu, Guoshi^*

来源：International Journal of Molecular Sciences, 2016, 17(6): 939.

DOI：10.3390/ijms17060939

• antioxidant
• melatonin
• mitochondria
• oocyte
• SNAT

The physiology of oocyte in vitro maturation remains elusive. Generally, the oocytes have a very low maturation rate under in vitro conditions. In the current study, we found that melatonin promotes the maturation of oocytes in which mitochondria play a pivotal role. It was identified that; (1) mitochondria are the major sites for melatonin synthesis in oocytes and they synthesize large amounts of melatonin during their maturation; (2) melatonin improves mitochondrial function by increased mtDNA copy, mitochondrial membrane potential (Delta Psi(m)) and mitochondrial distribution and ATP production in oocytes; (3) the meiotic spindle assembly is enhanced; (4) melatonin reduces ROS production and inhibits 8-oxodG formation, thereby protecting potential DNA mutation from oxidative damage. As a result, melatonin improves the quality of oocytes, significantly accelerates the developmental ability of IVF embryo. The results provide novel knowledge on the physiology of oocyte's maturation, especially under in vitro conditions.

• 出版日期2016-6
• 单位中国农业大学