ALU-PRIMED POLYMERASE CHAIN-REACTION FOR REGIONAL ASSIGNMENT OF 110 YEAST ARTIFICIAL CHROMOSOME CLONES FROM THE HUMAN X-CHROMOSOME - IDENTIFICATION OF CLONES ASSOCIATED WITH A DISEASE LOCUS

作者：NELSON DL^*; BALLABIO A; VICTORIA MF; PIERETTI M; BIES RD; GIBBS RA; MALEY JA; CHINAULT AC; WEBSTER TD; CASKEY CT

来源：Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(14): 6157-6161.

DOI：10.1073/pnas.88.14.6157

• DNA FINGERPRINTING
• DNA SEQUENCING
• LOWE SYNDROME
• SOMATIC CELL HYBRIDS

Over 400 yeast artificial chromosome (YAC) clones were isolated from the human X chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints. Polymerase chain reaction using Alu primers was applied to YAC clones in order to generate probes, to identify overlapping clones, and to derive "fingerprints" and sequence data directly from total yeast DNA. Several clones were identified in regions of medical interest. One set of three overlapping clones was found to cross a chromosomal translocation implicated in Lowe syndrome. The regional assignment of groups of YAC clones provides initiation points for further attempts to develop large cloned contiguous sequences, as well as material for investigation of regions involved in genetic diseases.

• 出版日期1991-7