A well-defined diblock copolymer of poly(ethylene oxide)-block-poly(4-vinylpyridine) for separation of basic proteins by capillary zone electrophoresis

作者：Liu Hang; Shi Ronghua; Wan Wenming; Yang Runmiao; Wang Yanmei^*

来源：Electrophoresis, 2008, 29(13): 2812-2819.

DOI：10.1002/elps.200700691

• capillary coating
• capillary zone electrophoresis
• diblock copolymer
• protein separation

A series of well-defined diblock copolymers, poly(ethylene oxide) -block-poly(4-vinylpyridine) (PEO-b-P4VP) used as physical coating of capillaries, were synthesized by atom transfer radical polymerization (ATRP). EOF measurement results showed that all synthesized PEO-b-P4VP diblock copolymer-coated capillaries in this report could suppress EOF effectively compared to the bare fused-silica capillaries, and efficient separations of basic proteins were achieved. The effects of the molecular weight of P4VP block in PEO-b-P4VP and buffer pH on the separation of basic proteins for CE were investigated in detail. Moreover, the relationships between morphologies of PEO-b-P4VP diblock copolymers in buffer, which were studied by transmission electron microscopy, and the separation efficiencies of basic protein with PEO-b-P4VP diblock copolymers coatings were discussed.

• 出版日期2008-7
• 单位中国科学技术大学