Design, synthesis and characterisation of mannosylated ovalbumin lipid core peptide self-adjuvanting vaccine delivery system

作者：Simerska Pavla^*; Ziora Zyta Maria; Fagan Vincent; Goodwin Daryn; Edrous Farrah; Toth Istvan

来源：Drug Delivery and Translational Research, 2014, 4(3): 246-255.

DOI：10.1007/s13346-013-0173-8

• Vaccine development
• Peptide synthesis
• Ovalbumin
• Glycosylation
• In vitro assay

Peptide-based vaccine delivery can be hampered by rapid peptidase activity and poor inherent immunogenicity. The self-adjuvanting lipid core peptide system(LCP) has been shown to confer improved stability and immunogenicity on peptide epitopes of group A Streptococcus, Chlamydia, hookworm, and malaria pathogens. However, various diseases, including cancer, still require targeted delivery of their vaccine candidates. For this reason, we have selected two model peptides (ovalbumin CD4(+) and/or CD8(+) T cell epitopes), and incorporated two or four copies of either epitope into our LCP vaccine. Optimised glycosylation of ovalbumin peptides yielded 46 % when microwave-assisted double coupling with 2 eq of carbohydrate derivative, activated by N, N-diisopropylethylamine and (O-benzotriazol-1-yl)-N, N, N', N'-tetramethyluronium hexafluorophosphate, was performed. All ovalbumin peptides were successfully synthesised and purified in 11-55 % yields by Fmoc- or Boc-chemistry using solid-phase peptide synthesis. The mannosylated ovalbumin peptides were nontoxic to human erythrocytes in haemolytic assay (<2% haemolysis) and showed increased (up to 20-fold) stability in plasma.

• 出版日期2014-6