Apoptosis Induction by OTA and TNF-alpha in Cultured Primary Rat Hepatocytes and Prevention by Silibinin

作者：Essid Ebtisam; Dernawi Yousef; Petzinger Ernst^*

来源：Toxins, 2012, 4(11): 1139-1156.

DOI：10.3390/toxins4111139

• ochratoxin A
• tumor necrosis factor alpha
• UV-C light
• hydrogen peroxide
• rat hepatocytes
• DNA ladder
• caspase-3
• apoptosis
• malondialdehyde
• silibinin

In cultures of primary rat hepatocytes, apoptosis occurred after application of 20 ng/mL tumor necrosis factor alpha (TNF-alpha). However, this was only in the presence of 200 ng/mL of the transcriptional inhibitor actinomycin D (ActD). This toxic effect was completely prevented in the presence of 25 mu g/mL soluble TNF-alpha receptor I (sTNFR I) in the supernatant of hepatocyte cell cultures. Apoptosis also occurred after application of 12.5 mu mol/L ochratoxin A (OTA). However, that was not prevented by up to 500 mu g/mL sTNFR I, indicating that TNF-alpha/TNFR I is not involved in OTA mediated apoptosis in hepatocytes. The antioxidative flavanolignan silibinin in doses from 130 to 260 mu mol/L prevented chromatin condensation, caspase-3 activation, and apoptotic DNA fragmentation that were induced by OTA, by 10 mmol/L hydrogen peroxide (H2O2) and by ultraviolet (UV-C) light (50 mJ/cm(2)), respectively. To achieve protection by silibinin, the drug was applied to the cell cultures for 2 h in advance. OTA stimulated lipid peroxidation on cultured immortalized rat liver HPCT cells, as was revealed by malondialdehyde (MDA) production. Lipid peroxidation occurred further by H2O2 and ActD/TNF-alpha incubation. These reactions were also suppressed by silibinin pretreatment. We conclude that the anti-apoptotic activity of silibinin against OTA, H2O2 and ActD/TNF-alpha is caused in vitro by the antioxidative effects of the flavanolignan. Furthermore, cytotoxicity of the pro-apoptotic toxins was revealed by MTT-test. When applied separately, ActD and TNF-alpha showed no cytotoxic effects after 24 h, but were cytotoxic if applied in combination. The used concentrations of OTA, H2O2 and the dose of UV-C caused a substantial decrease in viability within 36 h that was prevented mostly by silibinin. We conclude that silibinin is a potent protective compound against apoptosis and cytotoxicity caused by OTA and the investigated compounds.

• 出版日期2012-11