Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

作者：Jose Seena; Jayesh P; Mohandas A; Philip Rosamma; Singh I S Bright^*

来源：Marine Environmental Research, 2011, 71(3): 169-177.

DOI：10.1016/j.marenvres.2010.12.008

• Aquaculture
• Penaeus monodon
• Primary haemocyte culture
• Heavy metals
• Organophosphate pesticides
• Cytotoxicity
• Genotoxicity
• MTT assay
• Comet assay

Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos. Using mu assay 12 h IC50 values calculated were 31.09 +/- 16.27 mu M and 5.52 +/- 1.16 mu M for cadmium chloride and mercuric chloride and 59.94 +/- 52.30 mg l(-1) and 186.76 +/- 77.00 mg l(-1) for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.

• 出版日期2011-4